Many of the enzymes used industrially are produced by culturing bacteria. These investigations explore bacterial digestive enzymes and show how their activity can be measured.
Standard technique: Aseptic techniques
Tools covered: wire loop, pipettes, cotton wool plugs. Procedures covered: inoculating plates, inoculating slopes, inoculating cultures, using a wire loop, using a pipette, flaming necks of bottles and test tubes, and disinfecting surfaces.
Standard technique: Pouring an agar plate
Simple instructions for pouring an agar plate.
Digestion of starch: microbes
Compare the starch-digesting activity of two bacterial cultures with that of simple amylase solution.
Digestion of protein: microbes
Compare the protein-digesting activity of two microbial cultures with that of simple trypsin solution.
Standard technique: Incubating and viewing plates
Notes on handling plates safely: incubation temperatures, labelling plates, taping lids on, slowing growth and killing cultures.
Standard technique: Making up nutrient agars
Details of how to make up different culture media for culturing different microorganisms.